Antibodies are custom monoclonal antibodies in biomedical research and serve a range of applications, from detection to treatment. They are highly specific and sensitive to target proteins and cell markers, allowing researchers to decipher intricate biological mechanisms, identify novel targets, and clarify signaling pathways. They are essential for progressing medical research and enhancing patient care through precise diagnosis, efficient treatments, and the development of immunoassays like ELISA kits.
Choosing the right antibody is crucial for ensuring success in your project. Custom monoclonal antibodies (mAbs) are made from antibody-producing cells isolated from your own body, and can be used to detect and/or capture specific proteins of interest in biological samples. The process of creating mAbs requires a combination of expertise in antibody development, antigen design, hybridoma and recombinant technology, and mammalian expression systems.
Custom Rabbit Monoclonal Antibody Production: Why Researchers Prefer Rabbit mAbs
Immunologists use the hybridoma technique to generate custom antibodies by combining a gene encoding for an antibody with bacteriophage protein displays. This enables the bacteriophage protein to display the antibody gene, which immunologists then select from to isolate individual antibody-producing cells and generate a single clone of the desired antibody.
After generating a stable antibody cell line, immunologists can perform mass spectrometry to determine the antibody protein sequence. Many antibody production companies and facilities offer this service as an add-on to their monoclonal antibody generation services, or include it as part of the overall costs for producing the antibody. This sequenced molecule can then be compared to existing databases of monoclonal antibodies and polyclonal antibodies to confirm its identity.
